Lipid extraction according to modified Folch

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Description

  • liquid-liquid extraction using chloroform

Protocol

  • aqueous samples are used for lipid extraction including cell homogenates, homogenized tissue, plasma
  • to 0.8 volume parts aqueous sample 2 parts of methanol and subsequently 1 part of chloroform are added to give one phase (C:M:W=1:2:0.8)
  • the mixture is left for an hour at room temperature with periodical vortexing
  • 3 parts of chloroform and 0.7 parts of 0.2M KCl are added for phase separation (final composition C:M:W=2:1:0.75 v/v/v)
  • after vortexing the sample is centrifuged at 2000 rpm for 10 min
  • the lower chloroform phase containing the vast majority of lipids is isolated
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