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InstitutionConsorzio Mario Negri SudImage:Consorzio_mario_negri_sud_logo.gif
Principal investigator Daniela Corda

EMail: Daniela Corda

Telephone:+39(0)81 6132273 - +39(0)81 6132536
Country Italy
Beneficiary NumberP03
About usThe Consorzio Mario Negri Sud (CMNS), one of the four Mario Negri Institutes in Italy, is a

private, no-profit, research Institute located in Abruzzo and has a staff of around 210 people. The CMNS research groups have the common goal of applying basic cell biological approaches towards the identification of novel drug targets for cancer and genetic disorders. This is achieved through the pooling of a large range of technologies and expertise in cell biology and oncology including signal transduction, lipid metabolism, intracellular membrane traffic, and molecular oncogenesis. The CMNS is supported by a full range of equipment and techniques including light/confocal electron microscopy and electron tomography, histochemistry, fluorescent cell sorting, microinjection, mass spectrometry, proteomics, molecular biology, lipid analysis, biochemistry, cell biology, and bioinformatics. The CMNS offers a wide range of central core facilities, which include the Facility of Protein Analysis, of Light Microscopy and Bio-Imaging, of Electron Microscopy and Tomography, and of Basic Molecular Biology and an Animal House run by an Animal Care Unit. We have a long standing experience in model membrane studies, lipid analysis and metabolism in cells of different origins, mechanisms involved in membrane fusion and fission. The different approaches available will also be exploited to elucidate the role of the lipid transfer proteins FAPP2, GLTP and CERT in lipid droplet biogenesis and in the pathophysiology of lipid storage diseases. We have considerable experience in studying problems in the field of membrane trafficking and phosphoinositide signalling through the use of multidisciplinary approaches, including morphological analyses and biochemical (protein and lipid analysis) and molecular biology techniques. The studies on the role of lipid sensing nuclear receptors in hepato-biliary and gastrointestinal systems will be addressed with the aid of genetic manipulation; we are generating novel murine and cellular models for human lipid-related diseases. We are deciphering the molecular genetic programs controlled by the oxysterol receptor Liver X Receptor (LXR) and the bile acid receptor Farnesoid X Receptor (FXR). We have experience in the identification of novel nuclear receptor driven transcriptional cascades regulating lipid metabolism in the liver and intestine. The available tools and technologies include high throughput genotyping platforms, reporter assays, real time quantitative PCR, gain and loss of function adenoviruses for FXR and LXRalpha, FXR knockout mice and transgenic intestinal

FXR/LXRalpha mice.
ContributionsThe role of the partner is to analyze the dynamics of lipid droplets, using screening technologies

and monitoring their size, shape and number in hepatocytes, and the invovement of key elements of the endocytic and

exocytic pathways.
Member of TaskforcesTask Force IV, Task Force V, and Task Force IX
Member of WorkpackagesWP3

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