Staining of lipid droplets with Nile red and BODIPY 493/503

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Staining with Nile Red and BODIPY 493/503
Stock solutions
1. BODIPY 493/503 dissolved in ethanol to give a stock of 1mg/ml (can be stored in the dark at –20oC)
2. Nile red dissolved in DMSO to give a stock solution of 50ug/ml

Staining of lipid droplets
1. Aspirate off paraformaldehyde and rinse cells with PBS
2. Add PBS and shake for 5min
3. Aspirate and add PBS + Nile red (at 1:2000 dilution) or PBS + BODIPY 493/503 (at 1:1000 dilution)
4. Shake for 15min
5. Aspirate and wash 3 times with PBS
6. Mount cells in PBS



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