Template:Lipid mass spectrometry

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Sample preparation

  • liquid-liquid extraction, SPE ....
  • sample sovlent:
Material Material used Internal Standard(s) Internal Standard(s) added
Cultured cells 100µg protein IS1, IS2 xng each
Human plasma 10µl IS1, IS2 xng each

Instrumentation and method


  • Type:
  • Mode: isocratic or gradient
  • Solvent(s):
  • Gradient:
Time [min] Flow [ml/min]  % Solvent A  % Solvent B
0 0.2 100 0
1.0 0.2 50 50
1.5 0.2 100 0


if used

  • Type:
  • Temperature:


  • Type:
  • Injection volume:
  • Wash solvent:

Mass spectrometer

  • Type:
  • Ionization mode:
  • Ionization voltage:
  • Source temperature(s):
  • Collision gas:
  • Collision gas pressure:
  • MS/MS-conditions:
    • multiple reaction monitoring table
Analyte Precursor [m/z] Precursor [m/z] Collision energy [eV]
species 1 m/z 1 m/z 2 x
species 2 m/z 1 m/z 2 x

Data analysis and quantification

Data handling

  • chromatogram, spectrum and peak processing

Isotope correction

  • if necessary

Calibration and quantification

  • calibration type: matrix or external calibration by standard addition to sample matrix
  • species used for calibration
Species Material 1 Material 2
Species 1 range pmol range pmol
Species 1 range pmol range pmol

Method validation



Limit of detection


Sample data

Mass spectra



Please add categories as below for:

  • analytical techniques applied
  • lipid classes analyzed (see lipid class categories)
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