Ceramides - stratum corneum, free extractable - LC-MS - Farwanah et al.

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-	Please add categories for:	+	Please add categories as below for:
	* analytical techniques applied		* analytical techniques applied
	* lipid classes analyzed (see lipid class categories)		* lipid classes analyzed (see lipid class categories)
-	* Example: "[[Category:Flow injection analysis]]"	+
		+	[[Category:Lipid mass spectrometry]]

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Revision as of 17:04, 6 June 2008

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Contents 1 Sample preparation 2 Instrumentation and method 2.1 Pump 2.2 Autosampler 2.3 Mass spectrometer 3 Data analysis and quantification 3.1 Data handling 3.2 Isotope correction 3.3 Calibration and quantification 4 Method validation 4.1 Accuracy 4.2 Precision 4.3 LOD 4.4 LOQ 4.5 Recovery 5 Sample data 5.1 Mass spectra 5.2 Chromatogram 6 Reference

Sample preparation

• liquid-liquid extraction, SPE ....
• sample sovlent:

Material	Material used	Internal Standard(s)	Internal Standard(s) added
Cultured cells	100µg protein	IS1, IS2	xng each
Human plasma	10µl	IS1, IS2	xng each

Instrumentation and method

Pump

• Type:
• Mode: isocratic or gradient
• Solvent(s):
• Gradient:

Time [min]	Flow [ml/min]	% Solvent A	% Solvent B
0	0.2	100	0
1.0	0.2	50	50
1.5	0.2	100	0

Autosampler

• Type:
• Injection volume:
• Wash solvent:

Mass spectrometer

• Type:
• Ionization mode:
• Ionization voltage:
• Source temperature(s):
• Collision gas:
• Collision gas pressure:
• MS/MS-conditions:
  • multiple reaction monitoring table

Analyte	Precursor [m/z]	Precursor [m/z]	Collision energy [eV]
species 1	m/z 1	m/z 2	x
species 2	m/z 1	m/z 2	x

Data analysis and quantification

Data handling

• chromatogram, spectrum and peak processing

Isotope correction

• if necessary

Calibration and quantification

• calibration type: matrix or external calibration by standard addition to sample matrix
• species used for calibration

Species	Material 1	Material 2
Species 1	range pmol	range pmol
Species 1	range pmol	range pmol

Method validation

Accuracy

Precision

LOD

LOQ

Recovery

Sample data

Mass spectra

Chromatogram

Reference

• PubMed - Ref

Please add categories as below for:

• analytical techniques applied
• lipid classes analyzed (see lipid class categories)