Ceramides - stratum corneum, free extractable - LC-MS - Farwanah et al.
From LipidomicsWiki
(Difference between revisions)
(Mass spectrometric profiling of free extractable stratum corneum ceramides by means of LC/APCI-MS) |
|||
| Line 1: | Line 1: | ||
| - | How to use: | + | How to use: |
| - | + | ||
| - | + | ||
| - | + | ||
| + | *Click 'Edit' and copy the source code to clipboard | ||
| + | *Create new page/article and paste the source code into your new article. | ||
| + | *Modify as needed. | ||
| + | |||
| + | <br> | ||
== Sample preparation == | == Sample preparation == | ||
| - | Material: Palmoplantar stratum corneum scrapings from healthy volunteers | + | Material: Palmoplantar stratum corneum scrapings from healthy volunteers |
| - | Extraction: Chloroform–Methanol (1:1, v/v) | + | Extraction: Chloroform–Methanol (1:1, v/v) |
| - | + | ||
| - | + | ||
| + | == Instrumentation and method == | ||
=== Pump === | === Pump === | ||
| - | *Type: Spectra System P 4000 ( Thermo separations) | + | *Type: Spectra System P 4000 ( Thermo separations) |
*Mode: Gradient | *Mode: Gradient | ||
*Solvent(s): A: chloroform; B: chloroform, n- propanol, acetic acid (80:20:2) | *Solvent(s): A: chloroform; B: chloroform, n- propanol, acetic acid (80:20:2) | ||
| Line 45: | Line 46: | ||
=== Mass spectrometer === | === Mass spectrometer === | ||
| - | *Type: Finnigan LCQ classic (ion trap mass spectrometer) | + | *Type: Finnigan LCQ classic (ion trap mass spectrometer) |
| - | *Ionization mode: APCI, positive full scan mode | + | *Ionization mode: APCI, positive full scan mode |
*Source temperature(s): APCI heater: set to 500°C, heated capillary: 150°C<br> | *Source temperature(s): APCI heater: set to 500°C, heated capillary: 150°C<br> | ||
| + | <br> | ||
| + | <br> | ||
| - | + | <br> | |
| - | + | ||
| - | + | ||
== Reference == | == Reference == | ||
Revision as of 12:17, 2 September 2008
How to use:
- Click 'Edit' and copy the source code to clipboard
- Create new page/article and paste the source code into your new article.
- Modify as needed.
Contents |
Sample preparation
Material: Palmoplantar stratum corneum scrapings from healthy volunteers
Extraction: Chloroform–Methanol (1:1, v/v)
Instrumentation and method
Pump
- Type: Spectra System P 4000 ( Thermo separations)
- Mode: Gradient
- Solvent(s): A: chloroform; B: chloroform, n- propanol, acetic acid (80:20:2)
- Gradient:
| Time [min] | Flow [ml/min] | % Solvent A | % Solvent B |
|---|---|---|---|
| 0 | 1 | 100 | 0 |
| 15 | 1 | 0 | 100 |
Column
- Type: silica column LiChroCart 125-4 with Si 60 (5µm) (merck)
Mass spectrometer
- Type: Finnigan LCQ classic (ion trap mass spectrometer)
- Ionization mode: APCI, positive full scan mode
- Source temperature(s): APCI heater: set to 500°C, heated capillary: 150°C
Reference
Hany Farwanah , Peter Nuhnb, Reinhard Neubert , Klaus Raith. Normal-phase liquid chromatographic separation of stratum corneum ceramides with detection by evaporative light scattering and atmospheric pressure chemical ionization mass spectrometry. Analytica Chimica Acta 492 (2003) 233-239.
