Cholesterol / Cholesterylester - ESI-MS/MS - Liebisch et al.

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Contents

Abstract

In order to allow parallel analysis of free cholesterol (FC) and cholesteryl ester (CE) using ESI-MS/MS Liebisch et al. developed an acetyl chlorid derivatization method to convert FC to cholesterylacetate (CE 2:0). This method shows a precision and detection limit sufficient for routine analysis and due to the short run time of 1,3 min paired with an automated data analysis it allows high-throughput analysis.

Analysed Matrices

EDTA-plasma

Analytes

Sample Preparation

Analytical Method

ESI-MS-MS

Method Validation

Accuracy

Precision

LOD

LOQ

Recovery

Internal Standard

Reference

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