P02 UU

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{{Infobox_Institution
{{Infobox_Institution
-
|myInstitutionname=Utrecht University - Department of Biochemistry and Cell Biology
+
|myInstitutionname=Protagen AG - Stefan Müllner, Protagen AG, Dortmund
|myLogo=
|myLogo=
-
|myCountry=Netherland
+
|myCountry=Germany
-
|myBenefitnr=P02
+
|myBenefitnr=P20
|myAbout=
|myAbout=
-
|myContribution=The partner will be responsible for studying the mechanism and relevance of vitamin A deposition during hepatic stellate cell proliferation and differentiation for the known interaction with hepatocytes.
+
|myContribution=
 +
Protagen AG provides a unique technology platform addressing all aspects in modern protein
 +
science including:
 +
# 2-dimensional electrophoresis of lipid binding proteins
 +
A highly reproducible method for the differential display of membrane and plasma membrane
 +
proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel
 +
electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible
 +
differential display of membrane proteins was developed.
 +
# Identification of high affinity protein binders and binding proteins
 +
In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g.
 +
lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein
 +
macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein
 +
expression products produced in E.coli.
 +
# Protein Microarrays and Fast Selection of the best Antibody
 +
The set of binders used in the project for pull downs and other methods will be tested for
 +
specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false
 +
positive rate. This technology allows the quantitative analysis of the off-target binding
 +
partners and sensitivity/affinity testing.Specialised Microarrays for quantitative and statistical
 +
significant studies of protein lipid interaction will be produced employing the high-throughput
 +
capabilities at Protagen.
 +
# Posttranslational Modifications of Target Proteins
 +
Modiro® - a new software tool developed by Protagen allows the precise identification of all
 +
known and even hitherto unknown posttranslational protein modifications (PTM) in proteins
 +
and peptides. MS/MS spectra generated by machines from all manufactures can be
 +
analysed.
 +
# Automated Protein Identification Platform
 +
The latest generation of mass spectrometers by Bruker Daltonics were acquired in
 +
December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput
 +
and robotised workflow for proteome analysis is established and functional at
 +
Protagen. This platform is accessible for all project partners.
|myTaskforces=
|myTaskforces=
|myWorkpackages=
|myWorkpackages=
}}
}}

Revision as of 13:28, 3 June 2008

InstitutionProtagen AG - Stefan Müllner, Protagen AG, Dortmund
Principal investigator {{{myPrincipal_investigator}}}
Country Germany
Beneficiary NumberP20
About us
ContributionsProtagen AG provides a unique technology platform addressing all aspects in modern protein

science including:

  1. 2-dimensional electrophoresis of lipid binding proteins

A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible differential display of membrane proteins was developed.

  1. Identification of high affinity protein binders and binding proteins

In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein expression products produced in E.coli.

  1. Protein Microarrays and Fast Selection of the best Antibody

The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and sensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen.

  1. Posttranslational Modifications of Target Proteins

Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed.

  1. Automated Protein Identification Platform

The latest generation of mass spectrometers by Bruker Daltonics were acquired in December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput and robotised workflow for proteome analysis is established and functional at

Protagen. This platform is accessible for all project partners.
Member of Taskforces
Member of Workpackages

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