P02 UU
From LipidomicsWiki
Line 9: | Line 9: | ||
science including: | science including: | ||
# 2-dimensional electrophoresis of lipid binding proteins | # 2-dimensional electrophoresis of lipid binding proteins | ||
- | A highly reproducible method for the differential display of membrane and plasma membrane | + | A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible |
- | proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel | + | |
- | electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible | + | |
differential display of membrane proteins was developed. | differential display of membrane proteins was developed. | ||
# Identification of high affinity protein binders and binding proteins | # Identification of high affinity protein binders and binding proteins | ||
- | In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. | + | In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein |
- | lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein | + | |
- | macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein | + | |
expression products produced in E.coli. | expression products produced in E.coli. | ||
# Protein Microarrays and Fast Selection of the best Antibody | # Protein Microarrays and Fast Selection of the best Antibody | ||
- | The set of binders used in the project for pull downs and other methods will be tested for | + | The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and ensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen. |
- | specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false | + | |
- | positive rate. This technology allows the quantitative analysis of the off-target binding | + | |
- | partners and | + | |
- | significant studies of protein lipid interaction will be produced employing the high-throughput | + | |
- | capabilities at Protagen. | + | |
# Posttranslational Modifications of Target Proteins | # Posttranslational Modifications of Target Proteins | ||
- | Modiro® - a new software tool developed by Protagen allows the precise identification of all | + | Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed. |
- | known and even hitherto unknown posttranslational protein modifications (PTM) in proteins | + | |
- | and peptides. MS/MS spectra generated by machines from all manufactures can be | + | |
- | analysed. | + | |
# Automated Protein Identification Platform | # Automated Protein Identification Platform | ||
- | The latest generation of mass spectrometers by Bruker Daltonics were acquired in | + | The latest generation of mass spectrometers by Bruker Daltonics were acquired in December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput and robotised workflow for proteome analysis is established and functional at Protagen. This platform is accessible for all project partners. |
- | December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput | + | |
- | and robotised workflow for proteome analysis is established and functional at | + | |
- | Protagen. This platform is accessible for all project partners. | + | |
|myTaskforces= | |myTaskforces= | ||
|myWorkpackages= | |myWorkpackages= | ||
}} | }} |
Revision as of 13:29, 3 June 2008
Institution | Protagen AG - Stefan Müllner, Protagen AG, Dortmund | |
Principal investigator | {{{myPrincipal_investigator}}} | |
Country | Germany | |
Beneficiary Number | P20 | |
About us | ||
Contributions | Protagen AG provides a unique technology platform addressing all aspects in modern protein
science including:
A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible differential display of membrane proteins was developed.
In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein expression products produced in E.coli.
The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and ensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen.
Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed.
| |
Member of Taskforces | ||
Member of Workpackages |