P02 UU
From LipidomicsWiki
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Protagen AG provides a unique technology platform addressing all aspects in modern protein | Protagen AG provides a unique technology platform addressing all aspects in modern protein | ||
science including: | science including: | ||
- | + | * '''2-dimensional electrophoresis of lipid binding proteins''' | |
A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible | A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible | ||
differential display of membrane proteins was developed. | differential display of membrane proteins was developed. | ||
- | + | * '''Identification of high affinity protein binders and binding proteins''' | |
In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein | In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein | ||
expression products produced in E.coli. | expression products produced in E.coli. | ||
- | + | * '''Protein Microarrays and Fast Selection of the best Antibody''' | |
The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and ensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen. | The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and ensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen. | ||
- | + | * '''Posttranslational Modifications of Target Proteins''' | |
Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed. | Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed. | ||
- | + | * '''Automated Protein Identification Platform''' | |
The latest generation of mass spectrometers by Bruker Daltonics were acquired in December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput and robotised workflow for proteome analysis is established and functional at Protagen. This platform is accessible for all project partners. | The latest generation of mass spectrometers by Bruker Daltonics were acquired in December 2006 – Ultraflex III, HCT Ultra and MicroTOF-Q - by Protagen. In addition, a highthroughput and robotised workflow for proteome analysis is established and functional at Protagen. This platform is accessible for all project partners. | ||
|myTaskforces= | |myTaskforces= | ||
|myWorkpackages= | |myWorkpackages= | ||
}} | }} |
Revision as of 14:30, 3 June 2008
Institution | Protagen AG - Stefan Müllner, Protagen AG, Dortmund | |
Principal investigator | {{{myPrincipal_investigator}}} | |
Country | Germany | |
Beneficiary Number | P20 | |
About us | ||
Contributions | Protagen AG provides a unique technology platform addressing all aspects in modern protein
science including:
A highly reproducible method for the differential display of membrane and plasma membrane proteins, i.e. lipid associated proteins, based on the Large Gel 2-dimensional gel electrophoresis method (2DE) developed by Joachim Klose a method for highly reproducible differential display of membrane proteins was developed.
In order to identify high affinity protein ligands and other high-affinity binding molecules, e.g. lipids, aptamers, small molecules and nucleic acids Protagen employs a high content protein macroarray. The macroarrays contain His-tagged > 37.000 DNA sequenced human protein expression products produced in E.coli.
The set of binders used in the project for pull downs and other methods will be tested for specificity on protein Microarrays - UNIchip® - in order to significantly reduce the false positive rate. This technology allows the quantitative analysis of the off-target binding partners and ensitivity/affinity testing.Specialised Microarrays for quantitative and statistical significant studies of protein lipid interaction will be produced employing the high-throughput capabilities at Protagen.
Modiro® - a new software tool developed by Protagen allows the precise identification of all known and even hitherto unknown posttranslational protein modifications (PTM) in proteins and peptides. MS/MS spectra generated by machines from all manufactures can be analysed.
| |
Member of Taskforces | ||
Member of Workpackages |